PPT Slide

Relative B-factors or not?

The relative B-factor is principally a radiation damage correction

Do not use B-factor unless there is radiation damage, and the resolution is reasonably high (beyond 3Å)

-4

-2

0

2

-6

-4

-2

0

2

0

50

100

150

200

new crystal

No radiation damage

Bfactor off

Marked radiation damage

Bfactor on

k = kj exp[-2B sin2q/l2]

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