Choice of TLS groups and resolution
Choice: chains, domains, secondary structure elements,..more complex MD,...
Resolution: you have only 20 more parameters per TLS group.
Thioredoxin reductase 3 Å (Sandalova et al., (2001) PNAS, 98, 9533-8)
6 TLS groups (1 for each of 6 monomers in asu)