Analyse Data For MR
Look at the Experimental Data
You should do two things:
a) Create a Patterson map and search it for peaks. We expect a big peak at the origin (position 0,0,0) but if there is another big peak (perhaps about 0.25 the size of the origin peak) then perhaps there is translation between the molecules in the asymmetric unit and it will be more difficult to solve.
(The theory behind this is explained on the web site of Bernhard Rupp:
http://www-structure.llnl.gov/xray/101index.html
For more information, go to the section on Phasing Techniques on this website, and click on NCS with native Patterson maps)
b) Create a Wilson plot which is an indication of the self consistency of the data. Also find the average B-value of the data - this can be used to help the molecular replacement program.