PPT Slide

[localhost:~/test/muldlx1] harry% ls *mar2000

muldlx1_301.mar2000 muldlx1_307.mar2000 muldlx1_313.mar2000 muldlx1_319.mar2000

muldlx1_302.mar2000 muldlx1_308.mar2000 muldlx1_314.mar2000 muldlx1_320.mar2000

muldlx1_303.mar2000 muldlx1_309.mar2000 muldlx1_315.mar2000 muldlx1_321.mar2000

muldlx1_304.mar2000 muldlx1_310.mar2000 muldlx1_316.mar2000 muldlx1_322.mar2000

muldlx1_305.mar2000 muldlx1_311.mar2000 muldlx1_317.mar2000

muldlx1_306.mar2000 muldlx1_312.mar2000 muldlx1_318.mar2000

[localhost:~/test/muldlx1] harry% ipmosflm

************ Version 6.2.1 for Image plate and CCD data 23 April 2002 ***********

A.G.W. Leslie, MRC Laboratory Of Molecular Biology, HILLS ROAD, CAMBRIDGE CB2 2QH, UK

E-mail andrew@mrc-lmb.cam.ac.uk

New auto-indexing using DPS due to Ingo Steller Robert Bolotovsky and Michael Rossmann

(1998) J. Appl. Cryst. 30, 1036-1040

Original auto-indexing using REFIX due to Wolfgang Kabsch (Kabsch,W. (1993),

J.Appl.Cryst. 24,795-800.)

X-windows interface using xdl_view due to John Campbell (Daresbury Laboratory, UK.)

(Campbell,J.W. (1995) J. Appl. Cryst. 28, 236-242.

MOSFLM => image muldlx1_301.mar2000

MOSFLM => go

(Q)QOPEN: file opened on unit 1 Status: READONLY

Logical Name: muldlx1_301.mar2000 Filename: muldlx1_301.mar2000

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Crystal to detector distance of 250.00mm taken from image header

Wavelength of 1.54180A taken from image header

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Notes:

To run Mosflm, you need to have the program in your path and you need to know what it’s called on your system - often it’s “ipmosflm”.

If you run Mosflm and encounter difficulties and need help, make sure that you report which version of the program you are running - this is the first line of output to the screen, and also the first line in the standard log file, usually called mosflm.lp.

In this example, using a Mar image plate, the detector type is automatically detected so no DETECTOR keyword is necessary. For other detector types (e.g. ADSC, Rigaku, DIP, Mar CCD), default values are set up by using this keyword in conjunction with the detector type.

The GO keyword in conjunction with IMAGE tells the program to use the X-windows GUI for further processing.

The header information is read; in this case the crystal to detector distance, the wavelength, the pixel size of the detector, and the oscillation range of this image.