Beta-blip-mr

From Phaserwiki

β-Lactamase (BETA, 29kDa) is an enzyme produced by various bacteria, and is of interest because it is responsible for penicillin resistance, cleaving penicillin at the β-lactam ring. There are many small molecule inhibitors of BETA in clinical use, but bacteria can become resistant to these as well. Streptomyces clavuligerus produces beta-lactamase inhibitory protein (BLIP, 17.5kDa), which has been investigated as an alternative to small molecule inhibitors, as it appears more difficult for bacteria to become resistant to this form of BETA inhibition. The structures of BETA and BLIP were originally solved separately by experimental phasing methods. The crystal structure of the complex between BETA and BLIP has been a test case for molecular replacement because of the difficulty encountered in the original structure solution. BETA, which models 62% of the unit cell, is trivial to locate, but BLIP is more difficult to find. The BLIP component was originally found by testing a large number of potential orientations with a translation function search, until one solution stood out from the noise.

Tutorial 1

Search for blip alone.

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Tutorial 2

Search for beta and blip together.

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